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Find yourself

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Who am I? What is my purpose? These are very important questions, which require deep reflection. Most importantly be honest to yourself. Truly listen. It will protect you from giving into the stereotypes and expectations that the world imposes on you.

Spend time answering these questions NOW!!! You do not want to over commit to a path that is not for you. You may have heard someone confess ‘ I really did not want to be __________ but instead wanted to become ____? Sure you may achieve some level of success but if you don’t establish your identity you would not have fulfilled your true potential.

As you look back in the twilight of your career what will be your answers to these questions:

Will I do it over the same way again?

Have I wasted these years?

Now, more than ever we need bold, creative and innovative individuals. Our future depends on it.

JM

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How to write up the measurement of arginase activity lab

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Please read the ‘lab manual general guidelines’ document on myelearning for general instructions on how to write up the lab.

Here are some pointers for this particular lab.

ID#

Name of lab partner

Title:

Date: The date you did the lab

Aim: Be very specific – what were you measuring? Name of enzyme. What was the source of the enzyme?

Introduction:

This is a year 2 lab. You don’t have to define what is an enzyme or show how it works. Focus on the enzyme you are working with.

All enzymes will have an EC number. Start there.

What information does the digits in the EC number give?

Where in the body is the enzyme located? – organ as well as subcellular level.

State its reaction.

Show that it is part of the urea cycle.

Describe the principle of the assay used – rxn of urea with INPP. Show structures.

NOTE: You don’t have to talk about common laboratory equipment such as the spectrophotometer

Methodology and materials

  1. Materials/reagents must be listed with their concentrations (where possible); equipment/glassware not necessary
  2. Summary of method – continuous prose. NO POINT FORM
  3. Past tense

No tables.

The full method has to be given. Don’t say ‘ as seen in the manual’. You are expected to be able to use the method in your lab report and be able to do the lab without referring to the manual.

Results:

You must state your actual results. If there is something wrong with your values then you can go see Ms Fariyal and get some theoretical results to do your calculations. You will have to examine in your discussion why you think your results deviated from what was expected.

Number and title your tables and graphs.

In addition to the table for your calibration curve you will want to tabulate your results for the 2 mixtures.

For your calibration curve – state a title, clearly labeled axes, give a scale and it’s the best straight line through the origin. You can draw your graph on excel. Just set it up so that the straight line passes through the origin and get an equation of the line from excel so that you could work out the amount of urea in your samples.

Calculations:

This was discussed during your lab session.

Don’t forget to calculate your corrected absorbances.

In addition you are required to do up a flow chart for your calculations. This is NOT the flow chart you did for your lab session that broke down the procedure. This flow chart is different and shows all the dilutions and aliquots taken. This will let us know that you properly understood what you did in your calculations. So you will start with the liver giving its mass and work you way down to the final aliquot. Stating volumes along the way.

Discussion:

Compare your results obtained with other theoretical/experimental results.

Discuss how enzyme activity is measured and compare these techniques with the one you did for this lab.

State sources of error and precautions taken for this expt.

Why was the liver extract kept cold?

Why was the reaction carried out at 37oC ?

Why was MnSO4 used?

Why were the tubes foiled?

The use of perchloric acid.

References:

Use proper format as outlined in the Chicago manual of style 16th edition doc on myelearning.

Your information in the introduction and discussion must be cited and a list of your references given in alphabetical order at the end.

Diagrams and figures reproduced must be referenced.

Other things to note:

Remember its mL not ml

Attached a signed accountability statement form to your lab.

Submit your lab a week from when the lab was done. You are to submit your lab by 3:30pm to the biochem office Rm 217 in the old Nat Sci wing.

Submit your introduction and discussion in a word document to the Turnitin link on myelearning. This should be obvious but you will need to submit the same intro and discussion that you submitted from your hard copy to Turnitin. Failure to submit to Turnitin will result in loss of marks.

Plagiarism will result in the loss of marks and depending on the level of plagiarism you will get a zero grade and reported to the UWI examinations board.

Please direct all your questions to Ms Fariyal concerning the lab.

Thank you

JM

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Biochemistry extra credit assignment: Metabolomics

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Slide1

‘Omics’ industries:

  • Genomics
  • Transcriptomics
  • Proteomics
  • Metabolomics

These have become core technologies within almost every major academic or industrial research program around the world. When choosing a MSc. or PhD programme, you should ensure that they are covering some if not all of the above if you want to remain scientifically relevant and marketable in the future.

Metabolomics is a field of Life Science research that uses High Throughput technologies to identify and / or characterize all the small molecules or metabolites in a given cell, tissue or organism i.e the metabolome.

Metabolomics is a very young area of research as we now have the ability to measure and characterize very small molecules / metabolites in an organism.

Most common analytical techniques used in metabolomics are:

  • Gas chromatography – mas spectrometry (GC-MS)
  • Liquid chromatography – mas spectrometry (LC-MS)
  • Nuclear magnetic resonance (NMR) spectroscopy

Your mission:

Discuss how metabolomics studies have contributed to our understanding of disease mechanisms and the implications this will have on improving or possibly providing novel treatment strategies for these diseases.

Your reply could be in the form of a blog post reply or a video reply. If you are using text then a minimum of 250 words should be used and for videos the time limit is 5 mins. Please reference your material and include links to useful information.

Please state clearly in your reply:

  • The course code
  • Either your name or ID

Suggestion: Do up your reply as a post for your blog or video for your YouTube channel and then post that link up in the reply section of my blog. You are required to submit the text of your post or transcript of your video in a word document to the Turnitin link on myelearning. Only responses made in the reply section of this blog AND submitted to Turnitin will be marked.

Deadline: 30th Nov

A maximum of 10 marks will be awarded based on the quality of your answer.

Please be reminded that this is an extra credit assignment and not a compulsory requirement for this course.

Please let me know if you have any questions.

JM

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Biochemistry extra credit assignment: supplements

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Slide1

Supplements are everywhere. Dietary supplements and bodybuilding supplements – proteins, amino acids, cofactors, hormones, fat burners, antioxidants and multivitamins. These supplements are being sold based on what we know they do naturally in the human body. How efficacious these isolated, purified and sometimes synthesized supplements are, is a discussion for another time.

Your mission: 

I want you to chose any supplement and in the comments section of this blog post the following:

  1. A picture of the supplement
  2. State what the manufacturer is claiming this supplement is doing.
  3. By identifying the active ingredient(s) in the supplement justify the manufacturer’s claims using your biochemical knowledge of human metabolism.

Be creative with your responses. It could be in the form of a blog post reply or a video reply. If you are using text then a minimum of 250 words should be used and for videos the time limit is 5 mins.

Please state clearly in your reply:

  1. The course code
  2. Either your name or ID

Suggestion: Do up your reply as a post for your blog or video for your YouTube channel and then post that link up in the reply section of my blog. You are required to submit the text of your post or transcript of your video in a word document to the Turnitin link on myelearning. Only responses made in the reply section of this blog AND submitted to Turnitin will be marked.

Deadline: 28th Nov

A maximum of 10 marks will be awarded based on the quality of your answer.

Please be reminded that this is an extra credit assignment and not a compulsory requirement for this course.

Please let me know if you have any questions.

JM

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Principles of therapeutics and drug action pointers for BIOL 3363

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BIOL 3363 Medical Biotech

Topic: Principles of therapeutics and drug action

These are some things I want you all to look at when studying this topic

Know your definitions:

agonist

anaphylactic

antagonist

bioavailability – which mode of administration will give you 100% bioavailability

efficacy

permeability coefficient

pharmacodynamics

pharmacokinetics

potency

prophylactic

therapeutic

therapeutic index

tolerance

Differentiate between pharmacodynamics and pharmacokinetics


 

The mechanisms of different types of receptors

Ligand-gated channels – study the nicotinic receptor and the mechanism of action of acetylcholine (ACh) on it.

G protein coupled receptor – use the mechanism of action of glucagon as the example.

G protein coupled receptors tend to have the same general mechanism so we can use glucagon as the example. However different G proteins have different second messengers associated with them. I want you to name 4 second messengers.

Kinase linked receptors – use the mechanisms of action of insulin as the example. Note the difference between peptide hormones and steroid hormones

You should be able to describe the mechanism of action of cardiac gylycosides (digitalis).

NOTE: When talking about mechanisms of action you want to describe the structure of the receptor and the sequence of events that follow when agonist bind to receptor.

Please use the blog to share information with everyone by posting up comments, links and questions in the comments section below.

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Game birds can’t fly far

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tumblr_mduhteonOC1qko03zo1_500                  truffle-011

 

Did you know that game birds such as pheasants and quail are easily fatigued and can’t fly long distances? This is especially true when they are startled. A strategy used by many hunters. The reason being that that the flight muscles of game birds rely almost entirely on the metabolism of glycogen stored in the muscle.

It begs the question – How do migratory birds generate energy to fly long distances?

Love to know what you think.